ACCN2 Antibody (S271-44)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22409
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S271-44
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1
Localization
Cell Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ACCN2 Antibody (S271-44)
Western Blot: ACCN2 Antibody (S271-44) [NBP2-22409]
Western Blot: ACCN2 Antibody (S271-44) [NBP2-22409] - Western Blot analysis of Rat brain lysates showing detection of ACCN2 protein using Mouse Anti-ACCN2 Monoclonal Antibody, Clone S271-44 (NBP2-22409). Primary Antibody: Mouse Anti-ACCN2 Monoclonal Antibody (NBP2-22409) at 1:100, 1:250, 1:500, and 1:1000.Immunocytochemistry/ Immunofluorescence: ACCN2 Antibody (S271-44) [NBP2-22409]
Immunocytochemistry/Immunofluorescence: ACCN2 Antibody (S271-44) [NBP2-22409] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ACCN2 Monoclonal Antibody, Clone S271-44 (NBP2-22409). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ACCN2 Monoclonal Antibody (NBP2-22409) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ACCN2 Antibody (D) Composite.Immunocytochemistry/ Immunofluorescence: ACCN2 Antibody (S271-44) [NBP2-22409]
Immunocytochemistry/Immunofluorescence: ACCN2 Antibody (S271-44) [NBP2-22409] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.Applications for ACCN2 Antibody (S271-44)
Application
Recommended Usage
Western Blot
1:1000
Application Notes
1 ug/ml of ASIC1 Antibody was sufficient for detection of ASIC1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ACCN2
Long Name
Acid-sensing ion channel 1
Alternate Names
ASIC1, BNaC2
Gene Symbol
ASIC1
UniProt
Additional ACCN2 Products
Product Documents for ACCN2 Antibody (S271-44)
Product Specific Notices for ACCN2 Antibody (S271-44)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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