ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08557
![Western Blot: ACE/CD143 Antibody (ACE/3762)Azide and BSA Free [NBP3-08557]](https://resources.bio-techne.com/images/products/ACE-CD143-Antibody-ACE-3762-Azide-and-BSA-Free-Western-Blot-NBP3-08557-img0002.jpg "Western Blot: ACE/CD143 Antibody (ACE/3762)Azide and BSA Free [NBP3-08557]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2c Kappa Clone # ACE/3762
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa 652-788) of human ACE/CD143 protein (Uniprot: P12821)
Localization
Cell Surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2c Kappa
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07607).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free
Western Blot: ACE/CD143 Antibody (ACE/3762)Azide and BSA Free [NBP3-08557]
Western Blot: ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free [NBP3-08557] - Western Blot Analysis of human Kidney tissue lysate using ACE/CD143 Mouse Monoclonal Antibody (ACE/3762).![Immunohistochemistry-Paraffin: ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free [NBP3-08557]](https://resources.bio-techne.com/images/products/ACE-CD143-Antibody-ACE-3762-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08557-img0001.jpg "Immunohistochemistry-Paraffin: ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free [NBP3-08557]")
Immunohistochemistry-Paraffin: ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free [NBP3-08557]
Immunohistochemistry-Paraffin: ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free [NBP3-08557] - Formalin-fixed, paraffin-embedded human colon stained with ACE/CD143 Mouse Monoclonal Antibody (ACE/3762).
Western Blot Shows Human ACE/CD143 Specificity Using Knockout Cell Line.
Western blot shows lysates of SK-N-FI and ACE/CD143 knockout SK-N-FI cell line (KO). Nitrocellulose membrane was probed with ACE/CD143 Antibody (Catalog # NBP3-08557) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for ACE/CD143 (as indicated) in the parental SK-N-FI cell line, but is not detectable in knockout SK-N-FI cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).Applications for ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ACE/CD143
Long Name
Angiotensin I Converting Enzyme
Alternate Names
CD143, DCP1, Kininase II
Gene Symbol
ACE
Additional ACE/CD143 Products
Product Documents for ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free
Product Specific Notices for ACE/CD143 Antibody (ACE/3762) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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