Acetyl-CoA Carboxylase alpha/ACACA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55246

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Acetyl-CoA Carboxylase alpha/ACACA Antibodies »

Product Summary for Acetyl-CoA Carboxylase alpha/ACACA Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1925 and 1975 of human acetyl-Coenzyme A carboxylase alpha using the numbering given in entry NP_942131.1 (GeneID 31).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Acetyl-CoA Carboxylase alpha/ACACA Antibody

Immunoprecipitation: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NB100-55246]

Immunoprecipitation: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NB100-55246]

Immunoprecipitation: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NB100-55246] - Detection of Human ACC1/ACC-alpha on HeLa whole cell lysates using NB100-55246. ACC1/ACC-alpha was blotted using rabbit anti-ACC1/ACC-alpha antibody.

Applications for Acetyl-CoA Carboxylase alpha/ACACA Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Acetyl-CoA Carboxylase alpha/ACACA

In cells, excess metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA from acetyl CoA by Acetyl CoA carboxylase (ACC1) is the rate limiting step. The translocation of LCFA CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT1 and CPT2) and regulated by ACC2, the rate limiting step of mitochondrial fatty acid beta oxidation. Activities of ACC1 and 2 are regulated by their phosphorylation by 5' AMP activated protein kinase (AMPK). Diabetes deranges AMPK master switch and represses the ACC1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC alpha and is a cytosolic enzyme, enriched in liver, adipose, and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 has three main functions acting as a biotin carboxyl carrier protein, a biotin carboxylase, and a carboxyltransferase (catalytic activity). Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other, which is 59aa shorter than the predominant fat and liver isoform, exists in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoforms are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.

Long Name

Acetyl-Coenzyme A Carboxylase alpha

Alternate Names

ACACA, ACC1, ACCA, AcetylCoA Carboxylase alpha

Entrez Gene IDs

31 (Human)

Gene Symbol

ACACA

UniProt

Q13085

Additional Acetyl-CoA Carboxylase alpha/ACACA Products

Product Documents for Acetyl-CoA Carboxylase alpha/ACACA Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Acetyl-CoA Carboxylase alpha/ACACA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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