Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72039

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-72039
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3H4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Acetyl CoA synthetase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ACSS2(NP_061147) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

78.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free

Western Blot: Acetyl CoA synthetase Antibody (OTI3H4)Azide and BSA Free [NBP2-72039]

Western Blot: Acetyl CoA synthetase Antibody (OTI3H4)Azide and BSA Free [NBP2-72039]

Western Blot: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Acetyl CoA synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
Immunohistochemistry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]

Immunohistochemistry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]

Immunohistochemistry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039] - Staining of paraffin-embedded Human pancreas tissue using anti-Acetyl CoA synthetase mouse monoclonal antibody.
Flow Cytometry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]

Flow Cytometry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]

Flow Cytometry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Acetyl CoA synthetase antibody, and then analyzed by flow cytometry.

Applications for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl CoA synthetase

Acetyl CoA synthetase encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ACAS2, AceCS, acetate thiokinase, acetate-CoA ligase, Acetate--CoA ligase, Acetyl-CoA synthetase, acetyl-Coenzyme A synthetase 2 (ADP forming), acetyl-coenzyme A synthetase, cytoplasmic, ACSA, ACSACECS, Acyl-activating enzyme, acyl-CoA synthetase short-chain family member 2EC 6.2.1.1, cytoplasmic acetyl-coenzyme A synthetase, dJ1161H23.1, DKFZp762G026, EC 6.2.1

Gene Symbol

ACSS2

Additional Acetyl CoA synthetase Products

Product Documents for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.