Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72039
![Western Blot: Acetyl CoA synthetase Antibody (OTI3H4)Azide and BSA Free [NBP2-72039]](https://resources.bio-techne.com/images/products/Acetyl-CoA-synthetase-Antibody-OTI3H4-Azide-and-BSA-Free-Western-Blot-NBP2-72039-img0001.jpg "Western Blot: Acetyl CoA synthetase Antibody (OTI3H4)Azide and BSA Free [NBP2-72039]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3H4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human ACSS2(NP_061147) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
78.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free
Western Blot: Acetyl CoA synthetase Antibody (OTI3H4)Azide and BSA Free [NBP2-72039]
Western Blot: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Acetyl CoA synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti![Immunohistochemistry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]](https://resources.bio-techne.com/images/products/Acetyl-CoA-synthetase-Antibody-OTI3H4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72039-img0003.jpg "Immunohistochemistry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]")
Immunohistochemistry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]
Immunohistochemistry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039] - Staining of paraffin-embedded Human pancreas tissue using anti-Acetyl CoA synthetase mouse monoclonal antibody.![Flow Cytometry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]](https://resources.bio-techne.com/images/products/Acetyl-CoA-synthetase-Antibody-OTI3H4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72039-img0002.jpg "Flow Cytometry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]")
Flow Cytometry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039]
Flow Cytometry: Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free [NBP2-72039] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Acetyl CoA synthetase antibody, and then analyzed by flow cytometry.Applications for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Acetyl CoA synthetase
Alternate Names
ACAS2, AceCS, acetate thiokinase, acetate-CoA ligase, Acetate--CoA ligase, Acetyl-CoA synthetase, acetyl-Coenzyme A synthetase 2 (ADP forming), acetyl-coenzyme A synthetase, cytoplasmic, ACSA, ACSACECS, Acyl-activating enzyme, acyl-CoA synthetase short-chain family member 2EC 6.2.1.1, cytoplasmic acetyl-coenzyme A synthetase, dJ1161H23.1, DKFZp762G026, EC 6.2.1
Gene Symbol
ACSS2
Additional Acetyl CoA synthetase Products
Product Documents for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free
Product Specific Notices for Acetyl CoA synthetase Antibody (OTI3H4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...