Key Product Details

Species Reactivity

Validated:

Human, Bovine, Feline, Mouse (Negative), Rabbit, Rat (Negative)

Cited:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # HR2

Format

BSA Free

Concentration

1 mg/ml

View all Acetylcholinesterase/ACHE Antibodies »

Product Specifications

Immunogen

Purified human cerebellar acetylcholinesterase.

Reactivity Notes

No cross-reactivity with Frog.

Localization

Cell Membrane and Secreted

Specificity

This antibody does not detect butyrylcholinesterase (BChE).

Marker

Early Neuronal Development Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free [NB300-528]

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free [NB300-528]

Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in U251 Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free [NB300-528]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free [NB300-528]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Both normal and cancer biopsies of deparaffinized human Cerebellum tissue.
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free [NB300-528]

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free [NB300-528]

Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB300-528] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Hela Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
View 3 more images

Applications for Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:50

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500
Application Notes
IHC: Staining of AChE in human brain samples results in staining of nerve fibers and terminals. Cannot be used in WB to detect AChE. Not suitable for Flow.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetylcholinesterase/ACHE

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Alternate Names

ACHE, ARACHE, N-ACHE

Entrez Gene IDs

43 (Human)

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Acetylcholinesterase/ACHE Antibody (HR2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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