Acidic Calponin Antibody (4C4)
Novus Biologicals, part of Bio-Techne | Catalog # H00001266-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4C4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CNN3 (NP_001830.1, 1 a.a. ~ 329 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTHFNKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Acidic Calponin Antibody (4C4)
![Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]](https://resources.bio-techne.com/images/products/Acidic-Calponin-Antibody-4C4-Western-Blot-H00001266-M01-img0003.jpg "Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]")
Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]
Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01] - Analysis of CNN3 expression in transfected 293T cell line by CNN3 monoclonal antibody (M01), clone 4C4.Lane 1: CNN3 transfected lysate (Predicted MW: 36.4 KDa).Lane 2: Non-transfected lysate.![Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]](https://resources.bio-techne.com/images/products/Acidic-Calponin-Antibody-4C4-Western-Blot-H00001266-M01-img0002.jpg "Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]")
Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01]
Western Blot: Acidic Calponin Antibody (4C4) [H00001266-M01] - CNN3 monoclonal antibody (M01), clone 4C4. Analysis of CNN3 expression in HepG2.
Sandwich ELISA: Acidic Calponin Antibody (4C4) [H00001266-M01] - Detection limit for recombinant GST tagged CNN3 is 0.1 ng/ml as a capture antibody.
Applications for Acidic Calponin Antibody (4C4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Acidic Calponin
Alternate Names
calponin 3, acidic, Calponin, acidic isoform, calponin-3, dJ639P13.2.2 (acidic calponin 3)
Gene Symbol
CNN3
UniProt
Additional Acidic Calponin Products
Product Documents for Acidic Calponin Antibody (4C4)
Product Specific Notices for Acidic Calponin Antibody (4C4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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