Acidic Calponin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51972
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HGEYQDDYPRDYQYS corresponding to C-Terminus according to NP_001830.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Acidic Calponin Antibody
![Western Blot: Acidic Calponin Antibody [NBP1-51972]](https://resources.bio-techne.com/images/products/Acidic-Calponin-Antibody-Western-Blot-NBP1-51972-img0001.jpg "Western Blot: Acidic Calponin Antibody [NBP1-51972]")
Western Blot: Acidic Calponin Antibody [NBP1-51972]
Western Blot: Acidic Calponin Antibody [NBP1-51972] - NBP1-51972 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972]](https://resources.bio-techne.com/images/products/Acidic-Calponin-Antibody-Immunohistochemistry-Paraffin-NBP1-51972-img0002.jpg "Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972]")
Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972]
Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Acidic Calponin Antibody
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Peptide ELISA
Detection limit 1:64000
Western Blot
0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human duodenum, heart and kidney lysates (calculated MW of 36.4 kDa band according to NP_001830.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Lung (Bronchus).
IHC: Paraffin embedded Human Lung (Bronchus).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Acidic Calponin
Alternate Names
calponin 3, acidic, Calponin, acidic isoform, calponin-3, dJ639P13.2.2 (acidic calponin 3)
Entrez Gene IDs
1266 (Human)
Gene Symbol
CNN3
UniProt
Additional Acidic Calponin Products
Product Documents for Acidic Calponin Antibody
Product Specific Notices for Acidic Calponin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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