Aconitase 1 Antibody (22C10)

Western Blot: Aconitase 1 Antibody (22C10) [NBP3-26529] -

Western Blot: Aconitase 1 Antibody (22C10) [NBP3-26529] - Positive Western Blot detected in: Hela whole cell lysate.
All lanes: Aconitase 1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 100 kDa
Observed band size: 95-130 kDa

Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody (22C10) [NBP3-26529] -

Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody (22C10) [NBP3-26529] - Staining of HepG2 cell with Aconitase 1 Antibody (22C10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 511-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Aconitase 1 Antibody (22C10) [NBP3-26529] -

Flow Cytometry: Aconitase 1 Antibody (22C10) [NBP3-26529] - Overlay Peak curve showing HepG2 cells stained with Aconitase 1 Antibody (22C10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.