Aconitase 1 Antibody (JE63-98)

Flow Cytometry: Aconitase 1 Antibody (JE63-98) [NBP3-31993]

Flow Cytometry: Aconitase 1 Antibody (JE63-98) [NBP3-31993] - Flow cytometric analysis of HeLa cells labeling Aconitase 1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31993, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: Aconitase 1 Antibody (JE63-98) [NBP3-31993]

Western Blot: Aconitase 1 Antibody (JE63-98) [NBP3-31993] - Western blot analysis of Aconitase 1 on different lysates with Rabbit anti-Aconitase 1 antibody (NBP3-31993) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: 293T cell lysate (20 ug/Lane)
Lane 3: HCT 116 cell lysate (20 ug/Lane)
Lane 4: Mouse liver tissue lysate (40 ug/Lane)
Lane 5: Rat liver tissue lysate (40 ug/Lane)

Predicted band size: 98 kDa
Observed band size: 98 kDa

Exposure time: 5 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.