ACOT11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58936

Novus Biologicals, part of Bio-Techne
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NBP2-58936
NBP2-58936-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACOT11 Antibodies »

Product Summary for ACOT11 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: FLLLSDLRQRPEWDKHYRSVELVQQVDEDDAIYHVTSPALGGHTKPQDFVILASRRKPCDNGDPYVIALRSVTLPTHRETPEYRRGE

Reactivity Notes

Rat 89%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACOT11 Antibody

Western Blot: ACOT11 Antibody [NBP2-58936]

Western Blot: ACOT11 Antibody [NBP2-58936]

Western Blot: ACOT11 Antibody [NBP2-58936] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: ACOT11 Antibody [NBP2-58936]

Immunocytochemistry/ Immunofluorescence: ACOT11 Antibody [NBP2-58936]

Immunocytochemistry/Immunofluorescence: ACOT11 Antibody [NBP2-58936] - Staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: ACOT11 Antibody [NBP2-58936]

Immunohistochemistry-Paraffin: ACOT11 Antibody [NBP2-58936]

Immunohistochemistry-Paraffin: ACOT11 Antibody [NBP2-58936] - Staining in human duodenum and pancreas tissues using anti-ACOT11 antibody. Corresponding ACOT11 RNA-seq data are presented for the same tissues.
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Applications for ACOT11 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACOT11

ACOT11 (Acyl-CoA thioesterase11), also known as BFIT, is a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of this protein in mouse has been associated with obesity and alternative splicing results in two transcript variants encoding different isoforms.

Alternate Names

Acyl-CoA thioester hydrolase 11, acyl-CoA thioesterase 11BFIT1, Adipose-associated thioesterase, BFIT2, BFITDKFZp667O1916, Brown fat-inducible thioesterase, EC 3.1.2.-, EC 3.1.2.1, KIAA0707acyl-coenzyme A thioesterase 11, STARD14, START domain containing 14, THEAStAR-related lipid transfer (START) domain containing 14, THEM1, thioesterase superfamily member 1, thioesterase, adipose associated

Gene Symbol

ACOT11

Additional ACOT11 Products

Product Documents for ACOT11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACOT11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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