ACOT8 Antibody (3F1)
Novus Biologicals, part of Bio-Techne | Catalog # H00010005-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3F1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ACOT8 (NP_005460.2, 108 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SVRSVKAVQHGKPIFICQASFQQAQPSPMQHQFSMPTVPPPEELLDCETLIDQYLRDPNLQKRYPLALNRIAAQEVPIEIKPVNPSPLSQLQRMEPKQMFWVRARGYI
Specificity
Reacts with acyl-CoA thioesterase 8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for ACOT8 Antibody (3F1)
![Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]](https://resources.bio-techne.com/images/products/ACOT8-Antibody-3F1-Western-Blot-H00010005-M03-img0003.jpg "Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]")
Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]
Western Blot: ACOT8 Antibody (3F1) [H00010005-M03] - Analysis of ACOT8 expression in A-431 (Cat # L015V1).![Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]](https://resources.bio-techne.com/images/products/ACOT8-Antibody-3F1-Western-Blot-H00010005-M03-img0004.jpg "Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]")
Western Blot: ACOT8 Antibody (3F1) [H00010005-M03]
Western Blot: ACOT8 Antibody (3F1) [H00010005-M03] - Analysis of ACOT8 expression in transfected 293T cell line by ACOT8 monoclonal antibody (M03), clone 3F1. Lane 1: ACOT8 transfected lysate (Predicted MW: 35.9 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: ACOT8 Antibody (3F1) [H00010005-M03] - Detection limit for recombinant GST tagged ACOT8 is 1 ng/ml as a capture antibody.
Applications for ACOT8 Antibody (3F1)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against cell lysate in western blot and recombinant protein in ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ACOT8
Alternate Names
ACTEIII, acyl-CoA thioesterase 8HNAACTE, Choloyl-coenzyme A thioesterase, EC 3.1.2.27, hACTEIII, HIV-Nef-associated acyl-CoA thioesterase, hTEcholoyl-CoA hydrolase, long-chain fatty-acyl-CoA hydrolase, palmitoyl-CoA hydrolase, peroxisomal acyl-CoA thioesterase, peroxisomal acyl-CoA thioesterase 1, Peroxisomal acyl-coenzyme A thioester hydrolase 1, Peroxisomal long-chain acyl-CoA thioesterase 1, PTE-1, PTE1HIV-Nef associated acyl-CoA thioesterase, PTE2, PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8, Thioesterase II, thioesterase III
Entrez Gene IDs
10005 (Human)
Gene Symbol
ACOT8
UniProt
Additional ACOT8 Products
Product Documents for ACOT8 Antibody (3F1)
Product Specific Notices for ACOT8 Antibody (3F1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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