Acrosin Antibody (ACR-2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-419
![Immunocytochemistry/ Immunofluorescence: Acrosin Antibody (ACR-2) - BSA Free [NB500-419]](https://resources.bio-techne.com/images/products/Acrosin-Antibody-ACR-2-Immunocytochemistry-Immunofluorescence-NB500-419-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Acrosin Antibody (ACR-2) - BSA Free [NB500-419]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Porcine, Bovine (Negative), Canine (Negative), Human (Negative)
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # ACR-2
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for Acrosin Antibody (ACR-2) - BSA Free
Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Specificity
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Acrosin Antibody (ACR-2) - BSA Free
Immunocytochemistry/ Immunofluorescence: Acrosin Antibody (ACR-2) - BSA Free [NB500-419]
Immunocytochemistry/Immunofluorescence: Acrosin Antibody (ACR-2) [NB500-419] - Staining of capacitated boar sperm. Acrosome visualized using mouse monoclonal ACR-2 (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).Applications for Acrosin Antibody (ACR-2) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
1-12 ug/ml
Flow Cytometry
1-12 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:1000
Application Notes
Flow Cytometry: described in Ded et al., 2010 Immunocytochemistry: Membrane permeabilization (acetone) is essential. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Acrosin
Alternate Names
acrosin, ACRS, preproacrosin, proacrosin
Gene Symbol
ACR
Additional Acrosin Products
Product Documents for Acrosin Antibody (ACR-2) - BSA Free
Product Specific Notices for Acrosin Antibody (ACR-2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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