ACSL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89268

Novus Biologicals, part of Bio-Techne
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NBP1-89268
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACSL3 Antibodies »

Product Summary for ACSL3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSDIAVIM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACSL3 Antibody

Western Blot: ACSL3 Antibody [NBP1-89268]

Western Blot: ACSL3 Antibody [NBP1-89268]

Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ACSL3 antibody. Corresponding ACSL3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Western Blot: ACSL3 Antibody [NBP1-89268]

Western Blot: ACSL3 Antibody [NBP1-89268]

Western Blot: ACSL3 Antibody [NBP1-89268] - Analysis in control (vector only transfected HEK293T lysate) and ACSL3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268]

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268]

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP1-89268] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
View 3 more images

Applications for ACSL3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-89268 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACSL3

Long-chain-fatty-acid--CoA ligase 3, or ACSL3, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in both the biosynthesis of cellular lipids and degradation via beta-oxidation. Like other isozymes of this family, ALCS3 converts free long-chain fatty acids into fatty acyl-CoA esters. Specifically, ACSL3 mediates hepatic lipogenesis By similarity and has a mainly anabolic role in energy metabolism.

This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

ACS3EC 6.2.1.3, acyl-CoA synthetase long-chain family member 3, FACL3lignoceroyl-CoA synthase, fatty-acid-Coenzyme A ligase, long-chain 3, LACS 3, LACS3, Long-chain acyl-CoA synthetase 3, long-chain-fatty-acid--CoA ligase 3, PRO2194

Gene Symbol

ACSL3

Additional ACSL3 Products

Product Documents for ACSL3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ACSL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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