ACSL5 Antibody (CL0275) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44076

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0275

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ACSL5 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q9ULC5, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ACSL5 Antibody (CL0275) - Azide and BSA Free

ACSL5 Antibody (CL0275) - Azide and BSA Free Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP3-44076]

Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP3-44076]

Analysis in human duodenum and tonsil tissues using NBP3-44076 antibody. Corresponding ACSL5 RNA-seq data are presented for the same tissues.
ACSL5 Antibody (CL0275) - Azide and BSA Free Western Blot: ACSL5 Antibody [NBP3-44076]

Western Blot: ACSL5 Antibody [NBP3-44076]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
ACSL5 Antibody (CL0275) - Azide and BSA Free Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP3-44076]

Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP3-44076]

Staining of human endometrium shows strong membranous and cytoplasmic positivity in glandular cells.
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Applications for ACSL5 Antibody (CL0275) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACSL5

Long-chain-fatty-acid--CoA ligase 5, or ACSL5, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in both synthesis of cellular lipids and degradation via beta-oxidation. Like other isozymes of this family, ALCS5 converts free long-chain fatty acids into fatty acyl-CoA esters. Specifically, ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage.

This isozyme is highly expressed in uterus and spleen, and in trace amounts are found in normal brain. It also has significantly increased expression in malignant gliomas, where the protein functions in mediating fatty acid-induced glioma cell growth. Therefore, ALCS5 is thought to have a key role in the survival of glioma cells and may be a potential target for novel cancer treatments. Three transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

ACS2, ACS5FACL5 for fatty acid coenzyme A ligase 5, acyl-CoA synthetase long-chain family member 5, EC 6.2.1, EC 6.2.1.3, FACL5fatty acid coenzyme A ligase 5, fatty-acid-Coenzyme A ligase, long-chain 5, LACS 5, Long-chain acyl-CoA synthetase 5, long-chain fatty acid coenzyme A ligase 5, long-chain-fatty-acid--CoA ligase 5

Gene Symbol

ACSL5

Additional ACSL5 Products

Product Documents for ACSL5 Antibody (CL0275) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACSL5 Antibody (CL0275) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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