ACSL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2918

Novus Biologicals, part of Bio-Techne
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NB100-2918
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ACSL5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RTQIDSLYEHIQD corresponding to C-Terminus according to NP_057318.2, NP_976313.1, NP_976314.1.

Specificity

This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACSL5 Antibody

Western Blot Shows Human ACSL5 Specificity Using Knockout Cell Line.

Western Blot Shows Human ACSL5 Specificity Using Knockout Cell Line.

Western blot shows lysates of HCT 116 and ACSL5 knockout HCT 116 cell line (KO). Nitrocellulose membrane was probed with ACSL5 Antibody (Catalog # NB100-2918) followed by HRP-conjugated donkey anti-goat IgG Secondary Antibody. A specific band was detected for ACSL5 at approximately 64 kDa (as indicated) in the parental HCT 116 cell line, but is not detectable in knockout HCT 116 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Western Blot: ACSL5 Antibody [NB100-2918]

Western Blot: ACSL5 Antibody [NB100-2918]

Western Blot: ACSL5 Antibody [NB100-2918] - Staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ACSL5 Antibody [NB100-2918]

Immunohistochemistry-Paraffin: ACSL5 Antibody [NB100-2918]

Immunohistochemistry-Paraffin: ACSL5 Antibody [NB100-2918] - Staining of human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ACSL5 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in human spleen lysates (calculated MW of 76.0 kDa band according to NP_976313.1and NP_976314.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACSL5

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

ACS2, ACS5FACL5 for fatty acid coenzyme A ligase 5, acyl-CoA synthetase long-chain family member 5, EC 6.2.1, EC 6.2.1.3, FACL5fatty acid coenzyme A ligase 5, fatty-acid-Coenzyme A ligase, long-chain 5, LACS 5, Long-chain acyl-CoA synthetase 5, long-chain fatty acid coenzyme A ligase 5, long-chain-fatty-acid--CoA ligase 5

Entrez Gene IDs

51703 (Human)

Gene Symbol

ACSL5

UniProt

Q9ULC5 (Human)

Additional ACSL5 Products

Product Documents for ACSL5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACSL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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