Actin Antibody (S05-4F6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19993

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19993-100ul
NBP3-19993-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-4F6

Concentration

0.3 mg/ml

View all Actin Antibodies »

Product Summary for Actin Antibody (S05-4F6)

Immunogen

A synthetic peptide of human muscle Actin (Uniprot # P68133)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Actin Antibody (S05-4F6)

Western Blot: Actin Antibody (S05-4F6) [NBP3-19993]

Western Blot: Actin Antibody (S05-4F6) [NBP3-19993]

Western Blot: Actin Antibody (S05-4F6) [NBP3-19993] - Western blot detection of Actin in Hela, CHO-K1, C6, Rat Brain lysates using NBP3-19993 (1:1000 diluted). Predicted band size: 42kDa. Observed band size: 42kDa.
Immunohistochemistry-Paraffin: Actin Antibody (S05-4F6) [NBP3-19993]

Immunohistochemistry-Paraffin: Actin Antibody (S05-4F6) [NBP3-19993]

Immunohistochemistry-Paraffin: Actin Antibody (S05-4F6) [NBP3-19993] - Immunohistochemistry of Actin in paraffin-embedded Human colon cancer tissue using NBP3-19993 at dilution 1/50

Applications for Actin Antibody (S05-4F6)

Application
Recommended Usage

Immunohistochemistry

1/50

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Actin

Actin is a 43kDa filament protien that is ubiquitously expressed by all eukaryotic cells. This wide expression makes Actin an ideal "housekeeping gene", which may even account for up to 50% of total cellular protein. Actin microfilaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Actin antibodies are most commonly used as controls for protein standardization or in cytoskeletan or cell cycle research.

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, Alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Gene Symbol

ACTA1

Additional Actin Products

Product Documents for Actin Antibody (S05-4F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Actin Antibody (S05-4F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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