Actin Gamma 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-533

Novus Biologicals, part of Bio-Techne
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NB600-533
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Actin Gamma 1 Antibodies »

Product Specifications

Immunogen

The epitope recognized by this antibody maps to the N-terminus of human Actin Gamma 1 [UniProt# P63261]. The N-terminus of Actin Gamma 1 is highly conserved with Beta Actin and preliminary indications are that NB600-533 also recognizes Beta Actin (GeneID 60).

Reactivity Notes

Human and mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Actin Gamma 1 Antibody

Western Blot: Actin Gamma 1 Antibody [NB600-533]

Western Blot: Actin Gamma 1 Antibody [NB600-533]

Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (https://neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody [NB600-533]

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody [NB600-533]

Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody [NB600-533] - Detection of Actin Gamma 1 (Green) in Hela cells using NB600-533 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533]

Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533]

Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
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Applications for Actin Gamma 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:10-1:500

Simple Western

1:100
Application Notes
This Actin Gamma 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.Use in FLOW reported in scientific literature (PMID 28501006).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Actin Gamma 1

Actin, gamma 1 (ACTG1) is a member of the actin protein family, a highly conserved protein group with three main isoforms: alpha, beta and gamma. Alpha actins are mainly expressed in muscle tissues where they are a major constituent of the contractile apparatus, whereas beta and gamma actin proteins are widely expressed in most cell types where they are predominantly involved in cell motility and cytoskeleton maintenance. Actin gamma 1 is a cytoplasmic actin isoform usually found in nonmuscle cells.

Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.

Alternate Names

ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin

Entrez Gene IDs

71 (Human); 11465 (Mouse)

Gene Symbol

ACTG1

UniProt

P63261 (Human)

Additional Actin Gamma 1 Products

Product Documents for Actin Gamma 1 Antibody

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Product Specific Notices for Actin Gamma 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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