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Actin (Muscle Specific) Antibody (ACTA1/9105R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24312

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-24312

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Kappa Clone # ACTA1/9105R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Actin (Muscle Specific) Antibody (ACTA1/9105R) - Azide and BSA Free

Immunogen

Recombinant human Actin (Muscle Specific) protein

Reactivity Notes

Predicted to show a broad reactivity.

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Antibody without azide - store at -20 to -80C.

Applications for Actin (Muscle Specific) Antibody (ACTA1/9105R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml

Western Blot

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Actin (Muscle Specific)

Alternate Names

ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3

Gene Symbol

ACTA1

Additional Actin (Muscle Specific) Products

Product Documents for Actin (Muscle Specific) Antibody (ACTA1/9105R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Actin (Muscle Specific) Antibody (ACTA1/9105R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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