Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48154
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-HHF35-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48154-img0002.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # HHF35
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SDS extract of human myocardium.
Localization
Cytoplasmic
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Marker
Muscle Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-HHF35-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48154-img0003.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]")
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] - Human Leiomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).Applications for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.).
Optimal dilution for a specific application should be determined.
Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.).
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Actin (Muscle Specific)
Alternate Names
ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3
Gene Symbol
ACTA1
UniProt
Additional Actin (Muscle Specific) Products
Product Documents for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted
Product Specific Notices for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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