Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47662
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47662-img0005.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]")
![Western Blot: Actin (Muscle Specific) Antibody (MSA/953)Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Western-Blot-NBP2-47662-img0004.jpg "Western Blot: Actin (Muscle Specific) Antibody (MSA/953)Azide and BSA Free [NBP2-47662]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Rabbit
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MSA/953
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide common among human muscle actins
Localization
Cytoplasmic
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Marker
Muscle Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45274).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free
Western Blot: Actin (Muscle Specific) Antibody (MSA/953)Azide and BSA Free [NBP2-47662]
Western Blot: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47662-img0010.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]")
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662] - Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)![Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Flow-Intracellular-NBP2-47662-img0011.jpg "Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]")
Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]
Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662] - Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin format of this antibody.![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47662-img0006.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47662-img0007.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47662-img0008.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]](https://resources.bio-techne.com/images/products/Actin-Muscle-Specific-Antibody-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47662-img0009.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free [NBP2-47662]")
Applications for Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunohistochemistry-Paraffin
0.25 - 0.5 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Actin (Muscle Specific)
Alternate Names
ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3
Gene Symbol
ACTA1
UniProt
Additional Actin (Muscle Specific) Products
Product Documents for Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free
Product Specific Notices for Actin (Muscle Specific) Antibody (MSA/953) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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