ACTR1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1423

Novus Biologicals, part of Bio-Techne
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NB100-1423
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ACTR1A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YEEDGARSIHRKT corresponding to C-Terminus according to NP_005727.1.

Localization

Cytoplasmic

Specificity

This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACTR1A Antibody

Western Blot: ACTR1A Antibody [NB100-1423]

Western Blot: ACTR1A Antibody [NB100-1423]

Western Blot: ACTR1A Antibody [NB100-1423] - Analysis of ACTR1A in human kidney lysate using NB100-1423 at 2ug/ml (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: ACTR1A Antibody [NB100-1423]

Western Blot: ACTR1A Antibody [NB100-1423]

Western Blot: ACTR1A Antibody [NB100-1423] - Analysis of ACTR1A in human kidney lysate using NB100-1423 at 2ug/ml (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for ACTR1A Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 42.6 kDa band according to NP_005727.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACTR1A

The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.

Alternate Names

Actin-RPV, alpha-centractin, ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha), ARP1 actin-related protein 1 homolog A, centractin alpha (yeast), ARP1actin-RPV, centractin, Centrosome-associated actin homolog, CTRN1, FLJ52695, FLJ52800, FLJ55002

Entrez Gene IDs

10121 (Human); 54130 (Mouse)

Gene Symbol

ACTR1A

UniProt

P61163 (Human)

Additional ACTR1A Products

Product Documents for ACTR1A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ACTR1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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