ADAMTS13 Antibody (7B2Z8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16038

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B2Z8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ADAMTS13 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ADAMTS13 (Q76LX8). LSPGAPLKGRPPSPGFQRQRQRQRRAAGGILHLELLVAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPSLGAQFRVHLVKMVILTEPEGAPNITANLTSS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

154 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADAMTS13 Antibody (7B2Z8)

Western Blot: ADAMTS13 Antibody (7B2Z8) [NBP3-16038]

Western Blot: ADAMTS13 Antibody (7B2Z8) [NBP3-16038]

Western Blot: ADAMTS13 Antibody (7B2Z8) [NBP3-16038] - Western blot analysis of extracts of various cell lines, using ADAMTS13 antibody (NBP3-16038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry: ADAMTS13 Antibody (7B2Z8) [NBP3-16038]

Immunohistochemistry: ADAMTS13 Antibody (7B2Z8) [NBP3-16038]

Immunohistochemistry: ADAMTS13 Antibody (7B2Z8) [NBP3-16038] - Immunofluorescence analysis of mouse liver using ADAMTS13 Rabbit mAb (NBP3-16038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry: ADAMTS13 Antibody (7B2Z8) [NBP3-16038]

Immunohistochemistry: ADAMTS13 Antibody (7B2Z8) [NBP3-16038]

Immunohistochemistry: ADAMTS13 Antibody (7B2Z8) [NBP3-16038] - Immunofluorescence analysis of rat liver using ADAMTS13 Rabbit mAb (NBP3-16038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for ADAMTS13 Antibody (7B2Z8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADAMTS13

ADAMTS13 was first identified by its ability to cleave the ultra large aggregates of vWF into smaller forms in the serum. A thrombocytopenic disorder called thrombic thrombocytopenic purpura (TTR) was shown to be due to a deficiency in the cleavage of von Willebrands factor, and ADAMTS13 was identified as the enzyme responsible. ADAMTS13 cleaves the Tyr1605 to Met1606 bond in the A2 domain of vWF, a process stimulated by shear stress in the circulation and by binding to either platelet glycoprotein IBa or to heparin. The cleaved vWF leads to decreased platelet adhesion. An idiopathic form of TTR is found in patients with auto antibodies to ADAMTS13. The full length ADAMTS-1 sequence codes for a 1,427 amino acid protein, with a predicted mass of 153.6 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. The variants reported include 1,371, 1,340 and 344 amino acids, with predicted mass of 130.3, 144.5 and 36.7 kDa respectively. Many bands of varying sizes can be seen on Western blots, between 110-190 kDa, perhaps indicating differentially processed ADAMTS-13.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

Alternate Names

TTP, vWF-CP

Gene Symbol

ADAMTS13

Additional ADAMTS13 Products

Product Documents for ADAMTS13 Antibody (7B2Z8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADAMTS13 Antibody (7B2Z8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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