Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ADAMTS13 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 825 and 875 of human ADAM (a disintegrin-like and metalloprotease) metallopeptidase with thrombospondin type 1 motif, 13 using the numbering given in entry NP_620594.1 (GeneID 1109

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ADAMTS13 Antibody - BSA Free

Western Blot: ADAMTS13 Antibody [NB100-55262]

Western Blot: ADAMTS13 Antibody [NB100-55262]

Western Blot: ADAMTS13 Antibody [NB100-55262] - Detection of Human ADAMTS13 on HeLa whole cell lysates using NB100-55259, NB100-55260, NB100-55261 and NB100-55262. For blotting IPed ADAMTS13, goat anti-ADAMTS13 antibody was used.

Applications for ADAMTS13 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1 to 5 ug/mcl serum.

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ADAMTS13

ADAMTS13 was first identified by its ability to cleave the ultra large aggregates of vWF into smaller forms in the serum. A thrombocytopenic disorder called thrombic thrombocytopenic purpura (TTR) was shown to be due to a deficiency in the cleavage of von Willebrands factor, and ADAMTS13 was identified as the enzyme responsible. ADAMTS13 cleaves the Tyr1605 to Met1606 bond in the A2 domain of vWF, a process stimulated by shear stress in the circulation and by binding to either platelet glycoprotein IBa or to heparin. The cleaved vWF leads to decreased platelet adhesion. An idiopathic form of TTR is found in patients with auto antibodies to ADAMTS13. The full length ADAMTS-1 sequence codes for a 1,427 amino acid protein, with a predicted mass of 153.6 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. The variants reported include 1,371, 1,340 and 344 amino acids, with predicted mass of 130.3, 144.5 and 36.7 kDa respectively. Many bands of varying sizes can be seen on Western blots, between 110-190 kDa, perhaps indicating differentially processed ADAMTS-13.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

Alternate Names

TTP, vWF-CP

Entrez Gene IDs

11093 (Human)

Gene Symbol

ADAMTS13

UniProt

Q76LX8

Additional ADAMTS13 Products

Product Documents for ADAMTS13 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADAMTS13 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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