ADAMTS5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74343

Novus Biologicals, part of Bio-Techne
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NB100-74343
NB100-74343-0.025mg
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Key Product Details

Species Reactivity

Human, Porcine, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all ADAMTS5 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C K(636) N G Y Q S D A K G V K T F(649) of human ADAMTS5.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for ADAMTS5 Antibody

Application
Recommended Usage

Western Blot

1 ug/ml
Application Notes
WB: Detects an major band at approx. 68 kDa protein representing ADAMTS5 from porcine cartilage extract. On human recombinant ADAMTS5 WB: Detects a major band at approx. 85 kDa. Depending on the extent of C-terminal processing, several smaller bands may also be seen using this antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADAMTS5

ADAMTS5, also known as Implantin or Aggrecanase 2, is a member of the larger family of ADAM (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin-5 motif) was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines. Initial observations indicated a role for ADAMTS5 in aggrecan cleavage and cartilage destruction, especially in arthritis, and potentially a role in embryo implantation. ADAMTS5 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to efficiently cleave Aggrecan. In addition to the metalloprotease domain, ADAMTS5 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, a spacer domain, and two thrombospondin-1 like domains. ADAMTS5 is inhibited by the endogenous MMP inhibitors (TIMP1, 2, 3, and 4) but most efficiently by TIMP3. Unlike many of the ADAMs proteases, ADAMTS5 does not have a transmembrane domain, and is a secreted protein. Full length ADAMTS5 is a 930 amino acid protein with a predicted molecular mass is 101.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS5 a greater apparent molecular weight on reduced SDS PAGE gels. When ADAMTS5 is secreted, it is cleaved at the furin cleavage site (predicted molecular mass 73.2 kDa) and then further cleaved to generate a range of smaller forms.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 5

Alternate Names

ADAMTS11, ADMP-2, Aggrecanase 2

Entrez Gene IDs

11096 (Human)

Gene Symbol

ADAMTS5

UniProt

Q9UNA0

Additional ADAMTS5 Products

Product Documents for ADAMTS5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADAMTS5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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