ADAR Antibody (CL0176)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05500

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0176

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ADAR Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ADAR Antibody (CL0176)

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-05500 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.
Western Blot: ADAR Antibody (CL0176) [NBP3-05500]

Western Blot: ADAR Antibody (CL0176) [NBP3-05500]

Western Blot: ADAR Antibody (CL0176) [NBP3-05500] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ADAR Antibody (CL0176) [NBP3-05500]

Immunocytochemistry/ Immunofluorescence: ADAR Antibody (CL0176) [NBP3-05500]

Immunocytochemistry/Immunofluorescence: ADAR Antibody (CL0176) [NBP3-05500] - Staining of A549 cells using the Anti-ADAR monoclonal antibody, showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for ADAR Antibody (CL0176)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF fixation permeabilization use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAR

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Double-stranded RNA-specific adenosine deaminase

Alternate Names

ADAR1, DRADA, DSRAD, EC 3.5.4.37, G1P1, IFI-4, IFI4, K88DSRBP, p136

Gene Symbol

ADAR

Additional ADAR Products

Product Documents for ADAR Antibody (CL0176)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ADAR Antibody (CL0176)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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