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ADAR Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92918

Novus Biologicals, part of Bio-Techne
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NBP2-92918-0.02ml
NBP2-92918-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-299 of human ADAR1 (NP_001102.2). RPDGHSQGAPNSDPSLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADAR Antibody - BSA Free

ADAR Antibody

Western Blot: ADAR Antibody [NBP2-92918] -

Western Blot: ADAR Antibody [NBP2-92918] - analysis of 293F, using ADAR1 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.
ADAR Antibody

Immunohistochemistry-Paraffin: ADAR Antibody [NBP2-92918] -

Immunohistochemistry-Paraffin: ADAR Antibody [NBP2-92918] -Analysis of paraffin-embedded human colon using ADAR1 Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ADAR Antibody

ICC/IF-ADAR Antibody-NBP2-92918-

ICC/IF-ADAR Antibody-NBP2-92918- Analysis of HepG2 cells using ADAR1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Applications for ADAR Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ADAR

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Double-stranded RNA-specific adenosine deaminase

Alternate Names

ADAR1, DRADA, DSRAD, EC 3.5.4.37, G1P1, IFI-4, IFI4, K88DSRBP, p136

Gene Symbol

ADAR

Additional ADAR Products

Product Documents for ADAR Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ADAR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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