ADAT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85850

Novus Biologicals, part of Bio-Techne
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NBP1-85850
NBP1-85850-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ADAT1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IAQLCYEHYGIRLPKKGKPEPNHEWTLLAAVVKIQSPADKACDTPDKPVQVTKEVVSMGTGTKCIGQSKMRKNGDILNDSHAEVIARRSFQR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADAT1 Antibody

Western Blot: ADAT1 Antibody [NBP1-85850]

Western Blot: ADAT1 Antibody [NBP1-85850]

Western Blot: ADAT1 Antibody [NBP1-85850] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: ADAT1 Antibody [NBP1-85850]

Immunocytochemistry/ Immunofluorescence: ADAT1 Antibody [NBP1-85850]

Immunocytochemistry/Immunofluorescence: ADAT1 Antibody [NBP1-85850] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: ADAT1 Antibody [NBP1-85850]

Immunohistochemistry-Paraffin: ADAT1 Antibody [NBP1-85850]

Immunohistochemistry-Paraffin: ADAT1 Antibody [NBP1-85850] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for ADAT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAT1

ADAT1 is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. [provided by RefSeq]

Alternate Names

adenosine deaminase acting on tRNA, adenosine deaminase, tRNA-specific 1, EC 3.5.4, EC 3.5.4.-, hADAT1, tRNA-specific adenosine deaminase 1, tRNA-specific adenosine-37 deaminase

Entrez Gene IDs

23536 (Human)

Gene Symbol

ADAT1

UniProt

Q9BUB4 (Human)

Additional ADAT1 Products

Product Documents for ADAT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ADAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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