ADE2 Antibody (OTI1B4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02817
![Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]](https://resources.bio-techne.com/images/products/ADE2-Antibody-OTI1B4-Western-Blot-NBP2-02817-img0003.jpg "Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]")
![Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]](https://resources.bio-techne.com/images/products/ADE2-Antibody-OTI1B4-Flow-Cytometry-NBP2-02817-img0001.jpg "Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]")
![Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]](https://resources.bio-techne.com/images/products/ADE2-Antibody-OTI1B4-Western-Blot-NBP2-02817-img0005.jpg "Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI1B4
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
46.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ADE2 Antibody (OTI1B4)
Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817]
Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817]](https://resources.bio-techne.com/images/products/ADE2-Antibody-OTI1B4-Immunocytochemistry-Immunofluorescence-NBP2-02817-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817]")
Immunocytochemistry/ Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817]
Immunocytochemistry/Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ADE2.![Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]](https://resources.bio-techne.com/images/products/ADE2-Antibody-OTI1B4-Flow-Cytometry-NBP2-02817-img0002.jpg "Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]")
Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817]
Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADE2 antibody, and then analyzed by flow cytometry.Applications for ADE2 Antibody (OTI1B4)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ADE2
Alternate Names
ADE2, AIR carboxylase, AIRCADE2H1, DKFZp781N1372, MGC1343, MGC5024, multifunctional protein ADE2, PAIS, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, SAICAR synthetase
Entrez Gene IDs
10606 (Human)
Gene Symbol
PAICS
Additional ADE2 Products
Product Documents for ADE2 Antibody (OTI1B4)
Product Specific Notices for ADE2 Antibody (OTI1B4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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