Adenine Nucleotide Translocase 1 Antibody (3C1Q3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35061
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 3C1Q3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Adenine Nucleotide Translocase 1 (NP_001142.2).
Sequence:
LGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKG
Sequence:
LGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Adenine Nucleotide Translocase 1 Antibody (3C1Q3)
Immunoprecipitation: Adenine Nucleotide Translocase 1 Antibody (3C1Q3) [NBP3-35061] -
Immunoprecipitation: Adenine Nucleotide Translocase 1 Antibody (3C1Q3) [NBP3-35061] - Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug Adenine Nucleotide Translocase 1 antibody. Western blot was performed from the immunoprecipitate using Adenine Nucleotide Translocase 1 antibody at a dilution of 1:1000.Western Blot: Adenine Nucleotide Translocase 1 Antibody (3C1Q3) [NBP3-35061] -
Western Blot: Adenine Nucleotide Translocase 1 Antibody (3C1Q3) [NBP3-35061] - Western blot analysis of various lysates using [KO Validated] Adenine Nucleotide Translocase 1 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western Blot: Adenine Nucleotide Translocase 1 Antibody (3C1Q3) [NBP3-35061] -
Western Blot: Adenine Nucleotide Translocase 1 Antibody (3C1Q3) [NBP3-35061] - Western blot analysis of lysates from MCF7 cells, using [KO Validated] Adenine Nucleotide Translocase 1 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Applications for Adenine Nucleotide Translocase 1 Antibody (3C1Q3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunoprecipitation
0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Adenine Nucleotide Translocase 1
Alternate Names
AAC1, Adenine nucleotide translocator 1, ADP, ADP/ATP translocase 1, ANT 1, ANT1adenine nucleotide translocator 1 (skeletal muscle), ATP carrier protein 1, ATP carrier protein, heart/skeletal muscle, ATP carrier protein, heart/skeletal muscle isoform T1, heart/skeletal muscle ATP/ADP translocator, PEO2, PEO3, solute carrier family 25 (mitochondrial carrier; adenine nucleotidetranslocator), member 4, Solute carrier family 25 member 4, T1ANT
Gene Symbol
SLC25A4
Additional Adenine Nucleotide Translocase 1 Products
Product Documents for Adenine Nucleotide Translocase 1 Antibody (3C1Q3)
Product Specific Notices for Adenine Nucleotide Translocase 1 Antibody (3C1Q3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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