Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80562
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80562-img0010.jpg "Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
![Western Blot: Adenosine A2aR Antibody (7F6-G5-A2)Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Western-Blot-NBP2-80562-img0001.jpg "Western Blot: Adenosine A2aR Antibody (7F6-G5-A2)Azide and BSA Free [NBP2-80562]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Canine, Guinea Pig, Rabbit, Sheep
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 7F6-G5-A2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free
Immunogen
Full length recombinant human Adenosine A2a R. [UniProt# P29274]
Epitope
Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147).
Reactivity Notes
Sheep reactivity reported in scientific literature (PMID: 29791204). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free
Western Blot: Adenosine A2aR Antibody (7F6-G5-A2)Azide and BSA Free [NBP2-80562]
Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80562-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]
Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.![Immunohistochemistry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80562-img0006.jpg "Immunohistochemistry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
Immunohistochemistry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]
Immunohistochemistry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562] - Staining of human brain (putamen), antibody at 5 ug/mL. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80562-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80562-img0004.jpg "Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80562-img0005.jpg "Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80562-img0007.jpg "Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80562-img0008.jpg "Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]](https://resources.bio-techne.com/images/products/Adenosine-A2aR-Antibody-7F6-G5-A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80562-img0009.jpg "Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free [NBP2-80562]")
Applications for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 - 5 ug/ml
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100. Use reported in scientific literature (PMID 25836920)
Immunohistochemistry
1 - 5 ug/ml
Immunohistochemistry-Frozen
reported in scientific literature (PMID 9822147)
Immunohistochemistry-Paraffin
1 - 5 ug/ml
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Adenosine A2aR
Long Name
Adenosine A2A Receptor
Alternate Names
A2aR, Adenosine A2a R, ADORA2A, RDC8
Gene Symbol
ADORA2A
Additional Adenosine A2aR Products
Product Documents for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free
Product Specific Notices for Adenosine A2aR Antibody (7F6-G5-A2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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