Adenosine A2b R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-797

Novus Biologicals, part of Bio-Techne
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NB600-797
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rabbit

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Immunohistochemistry, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Adenosine A2b R Antibodies »

Product Specifications

Immunogen

Peptide with sequence CQADVKSGNGQ corresponding to C-Terminus according to NP_000667.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Adenosine A2b R Antibody

Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]

Immunocytochemistry/ Immunofluorescence: Adenosine A2b R Antibody [NB600-797]

Immunocytochemistry/Immunofluorescence: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml, showing cytoplasmic staining. The nuclear stain is DAPI (blue)
Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797]

Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797]

Immunohistochemistry-Paraffin: Adenosine A2b R Antibody [NB600-797] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: Adenosine A2b R Antibody [NB600-797]

Flow Cytometry: Adenosine A2b R Antibody [NB600-797]

Flow Cytometry: Adenosine A2b R Antibody [NB600-797] - analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line)
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Applications for Adenosine A2b R Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Immunocytochemistry/ Immunofluorescence

2-4ug/ml

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

3 ug/ml

Peptide ELISA

detection limit 1:64000
Application Notes
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.
Flow Cytometry: Flow cytometric analysis of Kelly cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenosine A2b R

Gene ID: 136 (human)

Long Name

Adenosine A2b Receptor

Alternate Names

Adenosine A2bR, ADORA2B

Entrez Gene IDs

136 (Human)

Gene Symbol

ADORA2B

UniProt

P29275 (Human)

Additional Adenosine A2b R Products

Product Documents for Adenosine A2b R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenosine A2b R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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