Adenosine Deaminase/ADA Antibody (4G4-1C6)

Novus Biologicals, part of Bio-Techne | Catalog # H00000100-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4G4-1C6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Adenosine Deaminase/ADA Antibodies »

Product Specifications

Immunogen

ADA (AAH40226.1, 1 a.a. ~ 363 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL

Specificity

ADA - adenosine deaminase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Adenosine Deaminase/ADA Antibody (4G4-1C6)

Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01]

Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01]

Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in Jurkat.
Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01]

Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01]

Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in HL-60.
ELISA: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01]

ELISA: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01]

ELISA: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - Detection limit for recombinant GST tagged ADA is 0.03 ng/ml as a capture antibody.

Applications for Adenosine Deaminase/ADA Antibody (4G4-1C6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Adenosine Deaminase/ADA

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.

Long Name

Adenosine Aminohydrolase

Alternate Names

ADA, ADA1

Entrez Gene IDs

100 (Human)

Gene Symbol

ADA

OMIM

102700 (Human)

UniProt

AAH40226 (Human)

Additional Adenosine Deaminase/ADA Products

Product Documents for Adenosine Deaminase/ADA Antibody (4G4-1C6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Adenosine Deaminase/ADA Antibody (4G4-1C6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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