Adenosine Deaminase/ADA Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77710

Novus Biologicals, part of Bio-Techne
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NBP1-77710-0.1mg
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Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

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Product Specifications

Immunogen

Adenosine Deaminase/ADA/ADA [Calf Spleen] (Uniprot: P56658)

Reactivity Notes

Cross reactivity against Adenosine Deaminase/ADA from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]

Scientific Data Images for Adenosine Deaminase/ADA Antibody [Biotin]

Western Blot: Adenosine Deaminase/ADA Antibody [Biotin] [NBP1-77710] - Mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used in whole cell lysate from Jurkat and Raji cells or 3% BSA.

Applications for Adenosine Deaminase/ADA Antibody [Biotin]

Application
Recommended Usage

ELISA

1:5000 - 1:20000

Immunoprecipitation

1:100

Western Blot

1:500 - 1:5000
Application Notes
This product has been tested by western blot. This product is suitable to be assayed against 1.0 ug of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:64,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Adenosine Deaminase/ADA

Adenosine deaminase is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and from the turnover of nucleic acids in tissues. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Inosine can then be deribosylated (removed from ribose) by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine. Mutations in the gene for adenosine deaminase causing it to not be expressed are one cause of severe combined immunodeficiency (SCID). Mutations causing it to be overexpressed are one cause of hemolytic anemia. There is some evidence that a different allelle (ADA2) may lead to autism. There are 2 isoforms of ADA: ADA1 and ADA2. ADA1 is found in most body cells, particularly lymphocytes and macrophages, where it is present not only in the cytosol but also as the ecto- form on the cell membrane attached to a protein called CD26. ADA2 has only been found in the macrophage where it co-exists with ADA1 where the two isoforms regulate the ratio of adenosine to deoxyadenosine to potentiate the killing of parasites. ADA2 is the predominant form present in human plasma and is increased in many diseases, particularly those associated with the immune system: for example rheumatoid arthritis, psoriasis and sarcoidosis. The plasma AD2 isoform is also increased in most cancers.

Long Name

Adenosine Aminohydrolase

Alternate Names

ADA, ADA1

Gene Symbol

ADA

UniProt

P56658 (Bovine)

Additional Adenosine Deaminase/ADA Products

Product Documents for Adenosine Deaminase/ADA Antibody [Biotin]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenosine Deaminase/ADA Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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