Adenylosuccinate Lyase Antibody (007)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89878

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Adenylosuccinate Lyase Antibodies »

Product Summary for Adenylosuccinate Lyase Antibody (007)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Adenylosuccinate Lyase (Uniprot#: P30566-1; Met1-Leu484).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Adenylosuccinate Lyase Antibody (007)

Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878]

Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878]

Western Blot: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDa Observed band size:55 kDa
Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Lyase Antibody (007) [NBP2-89878]

Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Lyase Antibody (007) [NBP2-89878]

Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ADSL monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Immunoprecipitation: Adenylosuccinate Lyase Antibody (007) [NBP2-89878]

Immunoprecipitation: Adenylosuccinate Lyase Antibody (007) [NBP2-89878]

Immunoprecipitation: Adenylosuccinate Lyase Antibody (007) [NBP2-89878] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-ADSL rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADSL rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDa

Applications for Adenylosuccinate Lyase Antibody (007)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylosuccinate Lyase

Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and , in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Adenylosuccinase, adenylosuccinate lyase, AMPS, ASase, ASL, EC 4.3.2, EC 4.3.2.2

Gene Symbol

ADSL

Additional Adenylosuccinate Lyase Products

Product Documents for Adenylosuccinate Lyase Antibody (007)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenylosuccinate Lyase Antibody (007)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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