Adenylosuccinate Lyase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87406

Novus Biologicals, part of Bio-Techne
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NBP1-87406
NBP1-87406-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Adenylosuccinate Lyase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RRICLAEAFLTADTILNTLQNISEGLVVYPKVIERRIRQELPFMATENIIMAMVKAGGSRQDCHEKIRVLSQQAASVVKQEGGDNDLIERIQVDAYFSPIHSQLDHLLDPSSFTGRASQQVQRFLEEEVYPLLKPYESVMKVKA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylosuccinate Lyase Antibody

Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406]

Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406]

Western Blot: Adenylosuccinate Lyase Antibody [NBP1-87406] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADSL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Lyase Antibody [NBP1-87406]

Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Lyase Antibody [NBP1-87406]

Immunocytochemistry/Immunofluorescence: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody [NBP1-87406] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
View 5 more images

Applications for Adenylosuccinate Lyase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylosuccinate Lyase

Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and , in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Adenylosuccinase, adenylosuccinate lyase, AMPS, ASase, ASL, EC 4.3.2, EC 4.3.2.2

Entrez Gene IDs

158 (Human)

Gene Symbol

ADSL

UniProt

P30566 (Human)

Additional Adenylosuccinate Lyase Products

Product Documents for Adenylosuccinate Lyase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adenylosuccinate Lyase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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