ADHFE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15297

Novus Biologicals, part of Bio-Techne
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NBP2-15297
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ADHFE1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADHFE1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%), Bovine (88%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADHFE1 Antibody

Western Blot: ADHFE1 Antibody [NBP2-15297]

Western Blot: ADHFE1 Antibody [NBP2-15297]

Western Blot: ADHFE1 Antibody [NBP2-15297] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ADHFE1 Antibody [NBP2-15297]

Immunocytochemistry/ Immunofluorescence: ADHFE1 Antibody [NBP2-15297]

Immunocytochemistry/Immunofluorescence: ADHFE1 Antibody [NBP2-15297] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: ADHFE1 protein stained by ADHFE1 antibody [N1C1] diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ADHFE1 Antibody [NBP2-15297]

Immunohistochemistry-Paraffin: ADHFE1 Antibody [NBP2-15297]

Immunohistochemistry-Paraffin: ADHFE1 Antibody [NBP2-15297] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Applications for ADHFE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ADHFE1

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM]

Alternate Names

ADH8, alcohol dehydrogenase, iron containing, 1, EC 1.1.99.24, Fe-containing alcohol dehydrogenase, HOTmitochondrial, MGC48605

Gene Symbol

ADHFE1

UniProt

Q8IWW8 (Human)

Additional ADHFE1 Products

Product Documents for ADHFE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ADHFE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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