Adiponectin/Acrp30 Antibody (19F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22450

Novus Biologicals, part of Bio-Techne
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NBP2-22450-0.025mg
NBP2-22450
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 19F1

Concentration

1 mg/ml

View all Adiponectin/Acrp30 Antibodies »

Product Specifications

Immunogen

Full length, recombinant, human adiponectin.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30357746).Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Adiponectin/Acrp30 Antibody (19F1)

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450]

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450]

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.
Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450]

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450]

Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Effect of whey peptides on 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated in the presence of 2.5 mg/mL WPI (whey protein isolate), WPH (whey peptide hydrolysate), BPI (BSA protein isolate) or BPH (BSA peptide hydrolysate). Immunoblotting and densitometric analysis of (A,D) adiponectin (n = 6). mRNA levels of (F) adiponectin (n = 8) were determined. * p < 0.05, **** p < 0.0001 vs. protein isolate controls. PS, protein stain. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32041341) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450]

Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450]

Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of Adiponectin was performed using 10% confluent 3T3-L1 cells differentiated with Adipogenesis supplement for 5 days.
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Applications for Adiponectin/Acrp30 Antibody (19F1)

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

2 - 4 ug/ml

Immunohistochemistry

1:20 - 100

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP2-22450 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adiponectin/Acrp30

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human)

Gene Symbol

ADIPOQ

UniProt

Q15848

Additional Adiponectin/Acrp30 Products

Product Documents for Adiponectin/Acrp30 Antibody (19F1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adiponectin/Acrp30 Antibody (19F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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