Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71422

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Adiponectin/Acrp30 Antibodies »

Product Summary for Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 19-244 of human ADIPOQ(NP_004788) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

Western Blot: Adiponectin/Acrp30 Antibody (OTI1H1)Azide and BSA Free [NBP2-71422]

Western Blot: Adiponectin/Acrp30 Antibody (OTI1H1)Azide and BSA Free [NBP2-71422]

Western Blot: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponecti
Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422]

Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422]

Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Adiponectin.
Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422]

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422]

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human Kidney tissue using anti-Adiponectin mouse monoclonal antibody.
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Applications for Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adiponectin/Acrp30

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Gene Symbol

ADIPOQ

Additional Adiponectin/Acrp30 Products

Product Documents for Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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