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AdipoR1 Antibody (SC69-04)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67631

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67631

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC69-04

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human AdipoR1 aa 5-54 / 375. (SwissProt: Q96A54 Human; SwissProt: Q91VH1 Mouse; Unigene:104556 Rat)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AdipoR1 Antibody (SC69-04)

Immunocytochemistry/ Immunofluorescence: AdipoR1 Antibody (SC69-04) [NBP2-67631]

Immunocytochemistry/ Immunofluorescence: AdipoR1 Antibody (SC69-04) [NBP2-67631]

Immunocytochemistry/Immunofluorescence: AdipoR1 Antibody (SC69-04) [NBP2-67631] - Staining ADIPOR1 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: AdipoR1 Antibody (SC69-04) [NBP2-67631]

Immunohistochemistry-Paraffin: AdipoR1 Antibody (SC69-04) [NBP2-67631]

Immunohistochemistry-Paraffin: AdipoR1 Antibody (SC69-04) [NBP2-67631] - Analysis of paraffin-embedded mouse liver tissue using anti-ADIPOR1 antibody. Counter stained with hematoxylin.
Flow Cytometry: AdipoR1 Antibody (SC69-04) [NBP2-67631]

Flow Cytometry: AdipoR1 Antibody (SC69-04) [NBP2-67631]

Flow Cytometry: AdipoR1 Antibody (SC69-04) [NBP2-67631] - Analysis of HepG2 cells with ADIPOR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Applications for AdipoR1 Antibody (SC69-04)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200
Application Notes
Use in WB reported in scientific literature (PMID:33581653)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AdipoR1

The adiponectin receptors, ADIPOR1 and ADIPOR2 (MIM 607946), serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM]

Long Name

Adiponectin Receptor 1

Alternate Names

ACDCR1, CGI45, PAQR1, TESBP1A

Gene Symbol

ADIPOR1

Additional AdipoR1 Products

Product Documents for AdipoR1 Antibody (SC69-04)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AdipoR1 Antibody (SC69-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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