ADK Antibody (4F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97934

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F8

Format

BSA Free

Concentration

1 mg/ml

View all ADK Antibodies »

Product Summary for ADK Antibody (4F8) - BSA Free

Immunogen

Recombinant human ADK (22-362aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ADK Antibody (4F8) - BSA Free

Western Blot: ADK Antibody (4F8)BSA Free [NBP1-97934]

Western Blot: ADK Antibody (4F8)BSA Free [NBP1-97934]

Western Blot: ADK Antibody (4F8) [NBP1-97934] - The cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: ADK Antibody (4F8) - BSA Free [NBP1-97934]

Immunocytochemistry/ Immunofluorescence: ADK Antibody (4F8) - BSA Free [NBP1-97934]

Immunocytochemistry/Immunofluorescence: ADK Antibody (4F8) [NBP1-97934] - Analysis in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: ADK Antibody (4F8) - BSA Free [NBP1-97934]

Flow Cytometry: ADK Antibody (4F8) - BSA Free [NBP1-97934]

Flow Cytometry: ADK Antibody (4F8) [NBP1-97934] - Flow cytometry analysis of ADK in A549 cell line, staining at 2- 5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Applications for ADK Antibody (4F8) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADK

Adenosine kinase (ADK) is the key regulator of adenosine metabolism. Because of the manifold receptor-dependent actions of adenosine, tight regulation of adenosine levels is crucial. The intracellular and extracellular pools of adenosine are in dynamic exchange by equilibrative and concentrative nucleoside transporters, so extracellular concentrations of adenosine are regulated by interplay of theses transporters with intracellular and extracellular enzymes of adenosine metabolism. Thus, the extracellular concentration of adenosine is enhanced by inhibition of equilibrative nucleoside transporters such as S-(4-nitrobenzyl)-6-thioinosine and cannabidiol and, stimulation of extracellular ATP breakdown and inhibition of intracellular adenosine removal.

Long Name

Adenosine Kinase

Alternate Names

AK

Gene Symbol

ADK

UniProt

NP_006712 (Human)

Additional ADK Products

Product Documents for ADK Antibody (4F8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ADK Antibody (4F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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