ADK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31802

Novus Biologicals, part of Bio-Techne
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NBP1-31802
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (96%), Monkey (99%), Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ADK Antibodies »

Product Summary for ADK Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADK. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (99%), Xenopus laevis (86%), Chicken (88%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADK Antibody

Western Blot: ADK Antibody [NBP1-31802]

Western Blot: ADK Antibody [NBP1-31802]

Western Blot: ADK Antibody [NBP1-31802] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ADK Antibody [NBP1-31802]

Immunocytochemistry/ Immunofluorescence: ADK Antibody [NBP1-31802]

Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP1-31802] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: ADK Antibody [NBP1-31802]

Western Blot: ADK Antibody [NBP1-31802]

Western Blot: ADK Antibody [NBP1-31802] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.

Applications for ADK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ADK

This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity.

Long Name

Adenosine Kinase

Alternate Names

AK

Gene Symbol

ADK

Additional ADK Products

Product Documents for ADK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ADK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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