Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Summary for ADK Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 312 to 362 of human Adenosine Kinase using the numbering given in entry NP_006712.2 (GeneID 132).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADK Antibody

Immunoprecipitation: ADK Antibody [NBP2-32189]

Immunoprecipitation: ADK Antibody [NBP2-32189]

Immunoprecipitation: ADK Antibody [NBP2-32189] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Adenosine Kinase antibody NBP2-32189 used for IP at 6 ug per reaction. Adenosine Kinase was also immunoprecipitated by rabbit anti-Adenosine Kinase antibody BL15848. For blotting immunoprecipitated Adenosine Kinase, NBP2-32189 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
ADK Antibody

Western Blot: ADK Antibody [NBP2-32189] -

Western Blot: ADK Antibody [NBP2-32189] - Wholecell lysate (50 ug) from HEK293T, LNCaP, and MCF-7 cellsprepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-ADK antibody usedfor WB at 0.4 ug/ml. Detection: Chemiluminescence withan exposure time of 10 seconds.
ADK Antibody

Western Blot: ADK Antibody [NBP2-32189] -

Western Blot: ADK Antibody [NBP2-32189] - : Wholecell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1,and BW5147.3 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-ADK antibody used for WB at 0.4 ug/ml. Detection:Chemiluminescence with an exposure time of 10 seconds

Applications for ADK Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ADK

Adenosine kinase (ATP:adenosine 5 prime phosphotransferase) is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma phosphate from ATP to adenosine, thereby serving as a potentially important regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of ADK could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as antiinflammatory agents. The encoded protein does not present any sequence similarities to other well characterized mammalian nucleoside kinases. In contrast, 2 regions were identified with significant sequence identity to microbial ribokinase and fructokinases and a bacterial inosine/guanosine kinase. Thus, ADK is a structurally distinct mammalian nucleoside kinase that appears to be akin to sugar kinases of microbial origin.

Long Name

Adenosine Kinase

Alternate Names

AK

Gene Symbol

ADK

UniProt

NP_006712.2 (Human)

Additional ADK Products

Product Documents for ADK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ADK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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