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ADNP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89236

Novus Biologicals, part of Bio-Techne
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NBP1-89236
NBP1-89236-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGSPFDPVFEVEPKISNDNPEEHVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQVSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEG

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35052632).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADNP Antibody

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Analysis in human testis and liver tissues using NBP1-89236 antibody. Corresponding ADNP RNA-seq data are presented for the same tissues.
Western Blot: ADNP Antibody [NBP1-89236]

Western Blot: ADNP Antibody [NBP1-89236]

Western Blot: ADNP Antibody [NBP1-89236] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP1-89236]

Immunocytochemistry/ Immunofluorescence: ADNP Antibody [NBP1-89236]

Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP1-89236] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.

Applications for ADNP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADNP

The activity-dependent neuroprotective protein (hADNP) gene is frequently amplified in many neoplasias, including breast, bladder, ovarian, pancreatic, and colon cancers. hADNP mRNA is abundantly expressed in distinct normal tissues, and high expression levels were encountered in malignant cells. hADNP is implicated in maintaining cell survival, perhaps through modulation of p53.

Long Name

Activity-dependent Neuroprotective Protein

Alternate Names

ADNP1

Gene Symbol

ADNP

Additional ADNP Products

Product Documents for ADNP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ADNP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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