ADP-Sugar Pyrophosphatase/NUDT5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00064
![Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]](https://resources.bio-techne.com/images/products/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0005.jpg "Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human ADP-Sugar Pyrophosphatase/NUDT5 (Uniprot#: Q9UKK9; Glu2-Phe219).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ADP-Sugar Pyrophosphatase/NUDT5 Antibody
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: ADP-Sugar Pyrophosphatase/NUDT5 knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa![Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]](https://resources.bio-techne.com/images/products/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Western-Blot-NBP3-00064-img0006.jpg "Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]")
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]
Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDa![Immunoprecipitation: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]](https://resources.bio-techne.com/images/products/ADP-Sugar-Pyrophosphatase-NUDT5-Antibody-Immunoprecipitation-NBP3-00064-img0004.jpg "Immunoprecipitation: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]")
Immunoprecipitation: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064]
Immunoprecipitation: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP3-00064] - ADP-Sugar Pyrophosphatase/NUDT5 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ADP-Sugar Pyrophosphatase/NUDT5 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 35 kDaApplications for ADP-Sugar Pyrophosphatase/NUDT5 Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ADP-Sugar Pyrophosphatase/NUDT5
Alternate Names
Nudix motif 5, YSA1, YSA1H
Gene Symbol
NUDT5
Additional ADP-Sugar Pyrophosphatase/NUDT5 Products
Product Documents for ADP-Sugar Pyrophosphatase/NUDT5 Antibody
Product Specific Notices for ADP-Sugar Pyrophosphatase/NUDT5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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