Adropin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26387

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.11 mg/ml

View all Adropin Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human Adropin (within residues 10-60). [Swiss-Prot# Q6UWT2]

Reactivity Notes

88% sequence identity with bovine protein.

Localization

secreted.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adropin Antibody - BSA Free

Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin Antibody [NBP1-26387] - Total protein from Human and Mouse brain was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-Adropin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin AntibodyBSA Free [NBP1-26387]

Western Blot: Adropin Antibody [NBP1-26387] - Western blot on Adropin overexpression lysate.

Applications for Adropin Antibody - BSA Free

Application
Recommended Usage

Western Blot

2 ug/ml
Application Notes
A band can be seen at 15 kDa in Western Blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.11 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adropin

Adropin (energy homeostasis-associated protein, ENHO) is a peptide hormone encoded by gene ENHO and its unique ORF generates a 76-residue long secretary peptide which is highly conserved in eutherian (placental) mammals. Adropin is abundantly expressed in liver/brain, and in mouse, hepatic expression as well as circulating adropin concentrations exhibits rapid regulation by fasting (inhibition) and refeeding (stimulation), suggesting regulation through metabolic status signals. Mouse obesity models further confirmed that adropin is involved in metabolic homeostasis and cardiovascular function. Diet-induced as well as genetically induced obesity has been found to be associated with decreased hepatic adropin expression and circulating adropin concentrations. Adropin-knockout mice exhibit increased adiposity and fasting triglycerides (TG), hepatic steatosis, insulin resistance, and increased propensity for impaired glucose tolerance with diet-induced obesity.

Alternate Names

C9orf165, ENHO, UNQ470

Entrez Gene IDs

375704 (Human)

Gene Symbol

ENHO

UniProt

Q6UWT2

Additional Adropin Products

Product Documents for Adropin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Adropin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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