AF10 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49867
Key Product Details
Species Reactivity
Validated:
Rat
Predicted:
Bovine (100%), Canine (100%), Human (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RLEDTTARFTNAN corresponding to internal region according to NP_001009569.1.
Epitope
RLEDTTARFTNAN - internal region
Specificity
This antibody is expected to recognize both reported isoforms (NP_004632.1; NP_001009569.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for AF10 Antibody
![Western Blot: AF10 Antibody [NBP1-49867]](https://resources.bio-techne.com/images/products/AF10-Antibody-Western-Blot-NBP1-49867-img0002.jpg "Western Blot: AF10 Antibody [NBP1-49867]")
Western Blot: AF10 Antibody [NBP1-49867]
Western Blot: AF10 Antibody [NBP1-49867] - Western blot analysis of AF10 in Rat Testis lysate (35ug protein in RIPA buffer) using NBP1-49867 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescenceApplications for AF10 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Experiments gave bands at approx. 140 kDa band and 60 kDa band in rat testis lysates. These bands correspond to earlier findings with different antibodies from other sources. This protein has a calculated MW of 109 kDa band according to human NP_004632.1, 107 kDa band according to human NP_001009569.1and 103 kDa band according to rat NP_001012162.1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AF10
Alternate Names
AF10MGC75086, ALL1-fused gene from chromosome 10 protein, DKFZp686E10210, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 10, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 10, protein AF-10, type I AF10 protein
Gene Symbol
MLLT10
UniProt
Additional AF10 Products
Product Documents for AF10 Antibody
Product Specific Notices for AF10 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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