AF10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90222

Novus Biologicals, part of Bio-Techne
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NBP1-90222
NBP1-90222-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AF10 Antibodies »

Product Summary for AF10 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TPGSVKSSSGSSVQSPQDFLSFTDSDLRNDSYSHSQQSSATKDVHKGESGSQEGGVNSFSTLIGLPSTSAVTSQPKSFENSPGDLGNSSLPTAGYKRAQTSGIEEETVKEKKRKGNKQSKHGPGRPKGNKNQENVSHLSVSSASPTSSV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AF10 Antibody

Immunocytochemistry/ Immunofluorescence: AF10 Antibody [NBP1-90222]

Immunocytochemistry/ Immunofluorescence: AF10 Antibody [NBP1-90222]

Immunocytochemistry/Immunofluorescence: AF10 Antibody [NBP1-90222] - Staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: AF10 Antibody [NBP1-90222]

Immunohistochemistry-Paraffin: AF10 Antibody [NBP1-90222]

Immunohistochemistry-Paraffin: AF10 Antibody [NBP1-90222] - Staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.

Applications for AF10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AF10

AF10 is a putative transcription factor containing a leucine zipper motif. A t(10;11)(p12;q23) chromosomal translocation present in a proportion of acute myeloid leukaemias results in expression of an AF10 (carboxy terminus) MLL (amino terminus) fusion protein.

Alternate Names

AF10MGC75086, ALL1-fused gene from chromosome 10 protein, DKFZp686E10210, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 10, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 10, protein AF-10, type I AF10 protein

Entrez Gene IDs

8028 (Human)

Gene Symbol

MLLT10

UniProt

P55197 (Human)

Additional AF10 Products

Product Documents for AF10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AF10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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