AF9 Antibody (HL1644) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25354
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1644
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human AF9. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AF9 Antibody (HL1644) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] -
Immunocytochemistry/Immunofluorescence: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] - AF9 antibody [HL1644] detects AF9 protein at nucleus and granule by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 stained by AF9 antibody [HL1644] (NBP3-25354) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Knockdown Validated: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] -
Knockdown Validated: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (NBP3-25354) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] -
Western Blot: AF9 Antibody (HL1644) - Azide and BSA Free [NBP3-25354] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody [HL1644] (NBP3-25354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for AF9 Antibody (HL1644) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AF9
Alternate Names
AF-9, AF9myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 3, ALL1-fused gene from chromosome 9 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 3, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, protein AF-9, YEATS domain-containing protein 3, YEATS3FLJ2035
Gene Symbol
MLLT3
Additional AF9 Products
Product Documents for AF9 Antibody (HL1644) - Azide and BSA Free
Product Specific Notices for AF9 Antibody (HL1644) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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