Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all AF9 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human ALL1 Fused Gene from Chromosome 9 Protein using the numbering given in entry NP_004520.1 (GeneID 4300).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AF9 Antibody

Western Blot: AF9 Antibody [NB100-1565]

Western Blot: AF9 Antibody [NB100-1565]

Western Blot: AF9 Antibody [NB100-1565] - Detection of Human AF9 on HeLa whole cell lysates using NB100-1565. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies NB100-1564, NB100-1566 and another AF9 Ab.
Immunohistochemistry-Paraffin: AF9 Antibody [NB100-1565]

Immunohistochemistry-Paraffin: AF9 Antibody [NB100-1565]

Immunohistochemistry-Paraffin: AF9 Antibody [NB100-1565] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AF9 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for AF9 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AF9

The human AF9 gene is one of the most common fusion partner genes with the MLL (mixed lineage leukemia) gene resulting in AF9/MLL translocations and leukemogenesis. AF9 is highly homologous to the gene product of ENL, another common MLL fusion partner gene. The function of AF9 is not known but through studies of ENL it is proposed to be a nuclear transactivating factor. AF9 is also known as ALL1 Fused Gene from Chromosome 9 Protein, MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocated 3, YEATS3, and FLJ2035.

Alternate Names

AF-9, AF9myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 3, ALL1-fused gene from chromosome 9 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 3, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, protein AF-9, YEATS domain-containing protein 3, YEATS3FLJ2035

Entrez Gene IDs

4300 (Human)

Gene Symbol

MLLT3

UniProt

NP_004520.1 (Human)

Additional AF9 Products

Product Documents for AF9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AF9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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