Afadin/AF-6 Antibody (3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11187

Novus Biologicals, part of Bio-Techne
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NBP3-11187
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunoassay, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3

Concentration

0.1 mg/ml

View all Afadin/AF-6 Antibodies »

Product Specifications

Immunogen

Monoclonal antibody 3 has been raised against peptide 1814-1829 aa of rat l-afadin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Afadin/AF-6 Antibody (3)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blot and Immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Fixation with PFA or acetone/methanol is recommended.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Afadin/AF-6

Afadin is an actin filament-binding protein that is involved in cell-cell adherens junctions (AJs) which directly interacts with the alpha herpus virus receptor, a member of the immunoglobulin supergene family. Afadin is colocalized with nectin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. L-Afadin has been isolated as an F-actin-binding protein from rat brain. L-afadin is ubiquitously expressed and specifically localized at zonula adherens (ZA) in epithelial cells and at cell-cell adherens junctions (AJs) in non-epithelial cells. L-afadin has a splice variant, named small intestine absorptive afadin (s-afadin) which is abundantly expressed in neural tissue. Monoclonal antibody 3 has been raised against peptide 1814-1829 aa of rat l-afadin. Monoclonal antibody 3 cross reacts with human, mouse and dog l-afadin.

Alternate Names

AF-6, AF6, AFAD, MLLT4

Gene Symbol

AFDN

Additional Afadin/AF-6 Products

Product Documents for Afadin/AF-6 Antibody (3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Afadin/AF-6 Antibody (3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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